Hepatocellular carcinoma test can be administered anywhere

But a team of researchers led by University of Utah chemical engineering and chemistry professor Marc Porter and U surgeon and professor Courtney Scaife has developed a rapid portable screening test for liver cancer (hepatocellular carcinoma) that doesn’t involve sending a specimen to a blood lab and cuts the wait time for results from two weeks to two minutes.

This new and inexpensive test — the team is working to lower the cost to about $3 per test — can be administered wherever the patient is, which will be particularly valuable in developing nations with little access to hospitals.

University of Utah chemical engineering and chemistry professor Marc Porter, left, and Nano Institute of Utah research associate Jennifer Granger, right, hold a spectrometer that works with a new rapid portable screening test for liver cancer they have developed that doesn’t involve sending a specimen to a blood lab and cuts the wait time for results from two weeks to two minutes. This inexpensive test can be administered wherever the patient is, which will be valuable for developing nations with little access to hospitals.

The U’s team — which includes Nano Institute of Utah research associate Jennifer Granger, chemical engineering doctoral student Alex Skuratovsky and U surgery assistant professor Jill E. Shea — published their research in the latest issue of Analytical Methods describing how the test works for alpha-fetoprotein, a widely-used marker of liver cancer.

Currently, testing for liver cancer involves lab-based blood tests and ultrasound imaging, both of which require traveling to major cities and can often cost more than a month’s salary in low- and middle-income countries. “If we can develop a rapid test that performs at a high level of clinical accuracy,” Porter says, “then we’ve got something that can have an impact on human lives.”

Researchers also believe the test can be easily modified to detect infectious diseases such as tuberculosis, malaria and dengue fever, a mosquito-borne tropical disease that can be life-threatening.

A small domino-sized cartridge holds a membrane for a new field test for liver cancer developed by researchers from the University of Utah. The test doesn’t involve sending a specimen to a blood lab and cuts the wait time for results from two weeks to two minutes. It can be administered wherever the patient is, which will be valuable for developing nations with little access to hospitals.

The test uses a small domino-sized plastic cartridge containing a paper membrane that selectively traps biomarkers (proteins specific to a certain disease) from biological fluids. A small droplet of blood, saliva, or urine, or even a teardrop, from the patient is dropped onto the membrane.

This is followed by the droplet of gold nanoparticles, which tags the biomarkers trapped in the membrane. If the biomarkers are present, a red spot appears, signaling the patient has the disease and should seek more testing and possible treatment.

“The concept is similar to a home pregnancy test, but instead of flowing laterally, it flows through the membrane,” says Granger, the lead author on the paper.

A handheld spectrometer manufactured by project collaborator B&W Tek, a Delaware-based manufacturer of mobile spectrometers, can analyse the membranes and measure how much of the biomarkers is present, which in the future could determine the severity of the disease or how a patient is responding to treatment.

The idea for the test is a spinoff of a similar test Porter developed eight years ago that astronauts on the International Space Station used to test the cleanliness of their drinking water. “This is a smarter offshoot of that,” says Porter, who also is the director of the Nano Institute of Utah.

Now that the team has proven the concept with liver cancer and built a prototype test kit, researchers plan to evaluate the technology in Mongolia in spring of 2019. The East Asian country has the highest rate of liver cancer in the world.