Component Management

Biofuel produced by microalgae

3rd March 2017
Enaie Azambuja
0

Scientists at Tokyo Institute of Technology have identified unique lysophosphatidic acid acyltransferases as being the central enzymes for triacylglycerol synthesis by oleaginous alga Nannochloropsis, thus uncovering the mechanisms of biofuel production in microalgae. In the modern society, energy generation heavily relies on fossil fuels, which, however, lead to environmental pollution and depletion of non-renewable resources.

Photosynthetic organisms such as plants and green algae can transform atmospheric carbon dioxide into carbon storage molecules, especially oils such as triacylglycerols (TAGs), which can be used as biofuels. In this context, microalgae provide advantages of high oil content and growth in extreme environments, including high salinity, temperature, or pH.

Nannochloropsis is a genus of microalgae which can accumulate TAGs up to 50% of dry weight; however, the mechanisms underlying their oleaginous trait are largely unknown. Scientists from Tokyo Institute of Technology lead by Professor Hiroyuki Ohta have addressed this problem by investigating lipid metabolism in Nannochloropsis oceanica.

TAGs are synthesised in the extraplastidic Kennedy pathway through sequential addition of three fatty acyl moieties to the glycerol backbone. Among the participating enzymes, the scientists focused on four lysophosphatidic acid acyltransferases (LPATs 1-4) responsible for the addition of fatty acids at position 2.

They found that phylogenetically, LPAT1 and LPAT2 belong to different subfamilies, while LPAT3 and LPAT4 have a close evolutionary relationship. Accordingly, these enzymes appeared to have distinct functional activities as revealed by using mutant strains of N. oceanica lacking either one or two of the four LPATs. Thus, LPAT1 was found to mainly participate in the synthesis of membrane lipids, while LPAT4 was responsible for TAG biosynthesis, and LPAT2 and LPAT3 contributed to both processes.

LPATs were labeled with fluorescent tags and their intracellular location was examined using confocal microscopy. While LPAT1 and LPAT2 showed a typical ER localisation pattern, LPAT3 and LPAT4 were observed specifically at the perimeter of lipid droplets (LDs) (Figure 2), which was likely due to the presence of long (30-40 residues) hydrophobic domains in their structures that enable anchoring to the LD surface.

Based on their results, the scientists suggest that for LD formation, LPAT2 is mainly involved in the initial TAG synthesis in the ER, and LPAT3 and LPAT4 localise on LD surface in the periphery and contribute to further growth of LDs.

Thus, the study of Professor Ohta and his colleagues provide direct evidence that the oleaginous trait of Nannochloropsis is supported by LPATs in the perimeter of LDs.

Featured products

Upcoming Events

View all events
Newsletter
Latest global electronics news
© Copyright 2024 Electronic Specifier